Mike Sims
April 1st, 2012, 07:28 PM
Zoo in a Dewdrop (http://vimeopro.com/user8310268/uwol-22#/video/39341914)
I used to take a lot of photographs through microscopes- not so much for my own research, but because I had acquired the knack, frequently out of “professional courtesy” for “departmental colleagues” in aid of various reports and papers. While I was in graduate school I took several classes in oceanic plankton. A while back I decided that I would like to collect video of many of the strange looking creatures which participate in the planet’s greatest migration (greatest in numbers, greatest in biomass, greatest in frequency…)- the daily migration of plankton to and from the depths. I acquired a microscope (AmScope model #T940B). It is not on a par with the research grade instruments I used to use but is mechanically and, somewhat, optically sound and was within my reduced means. Shortly afterwards UWOL 19 “Motion” was held. I had thought to do a video on the Kingdom Protista, the main division of which is based on the means of locomotion. (Protist ~= protozoa. In this video I used an even older name, animalcules, which was coined in 1683 by Antonie van Leeuwenhoek who discovered protists but did not invent the microscope.) Unfortunately I became ill and wasn’t able to shoot even a single frame for UWOL 19 (and I still haven‘t started on plankton yet either!). When the theme for UWOL 22 was announced I revived and almost completely revised the idea.
The micro clips were shot on a Canon 550D (T2i) coupled to the third axis of the microscope with an EF to M42 adapter and a Meade camera adapter for telescopes. An eyepiece pointer adapter was between the Meade adapter and the microscope. The camera was used at prime focus, with simple projection (akin to using close-up rings), and with eyepiece projection. The projection eyepiece was an 18mm Meade Plössl. Microtechniques used include vital staining, dark field (which trades resolution for contrast) and Rheinberg illumination. Scale was measured with a stage micrometer.
The macro clips were shot on a Canon XLH1a recording to an Atomos Samurai through a Blackmagic Design Analogue to SDI Mini Converter. The lens used was a Canon EF 100-400L with a Canon 500D diopter. Specimens were in a Carolina Micro Aquarium (#131016).
Subjects were easily acquired by “fishing” in my 23 year old 50 gallon Leiden-style aquarium. It is truly a “micro-menagerie”.
The “reverse UWOL” clip was shot with a Canon SD 1400 IS in a WP-DC37 underwater housing on a ProAm DVC200 crane (which was recommended by Per Johan Naesje- Thanks Per Johan!). I was not in the river but the tripod was. (Good thing because wading is illegal!) The sky was completely blown out and was rescued by the non replacement technique discussed at the end of Andrew Kramer’s Video Copilot Tutorial #42- Advanced Sky Replacement. I’m pleased how well the footage from the little camera cut against the other footage. I believe this is the only clip at normal speed.
Slow motion was done by:
1) Smooth Slow Record (120 fps) on a Sony HDR-HC9 conformed to 24P.
2) 60P conformed to 24P.
3) 30i conformed to 24P.
4) any of the above further slowed with Kronos.
Combination of 1 and 4 was least effective due to the reduced resolution. The fast motion was simply done in Premier.
Audio was a major concern for me. No Foley this time. What kind of sound does a paramecium make? I hope the choice of music worked.
Would any of these creatures really exist in a dewdrop? Obviously some are too large and the title is somewhat “creative license”, however, protists dry up and encyste and are quick to inhabit the most ephemeral of bodies of water. Even dewdrops…
As usual, all questions, comments and critique is welcomed. Thanks.
I used to take a lot of photographs through microscopes- not so much for my own research, but because I had acquired the knack, frequently out of “professional courtesy” for “departmental colleagues” in aid of various reports and papers. While I was in graduate school I took several classes in oceanic plankton. A while back I decided that I would like to collect video of many of the strange looking creatures which participate in the planet’s greatest migration (greatest in numbers, greatest in biomass, greatest in frequency…)- the daily migration of plankton to and from the depths. I acquired a microscope (AmScope model #T940B). It is not on a par with the research grade instruments I used to use but is mechanically and, somewhat, optically sound and was within my reduced means. Shortly afterwards UWOL 19 “Motion” was held. I had thought to do a video on the Kingdom Protista, the main division of which is based on the means of locomotion. (Protist ~= protozoa. In this video I used an even older name, animalcules, which was coined in 1683 by Antonie van Leeuwenhoek who discovered protists but did not invent the microscope.) Unfortunately I became ill and wasn’t able to shoot even a single frame for UWOL 19 (and I still haven‘t started on plankton yet either!). When the theme for UWOL 22 was announced I revived and almost completely revised the idea.
The micro clips were shot on a Canon 550D (T2i) coupled to the third axis of the microscope with an EF to M42 adapter and a Meade camera adapter for telescopes. An eyepiece pointer adapter was between the Meade adapter and the microscope. The camera was used at prime focus, with simple projection (akin to using close-up rings), and with eyepiece projection. The projection eyepiece was an 18mm Meade Plössl. Microtechniques used include vital staining, dark field (which trades resolution for contrast) and Rheinberg illumination. Scale was measured with a stage micrometer.
The macro clips were shot on a Canon XLH1a recording to an Atomos Samurai through a Blackmagic Design Analogue to SDI Mini Converter. The lens used was a Canon EF 100-400L with a Canon 500D diopter. Specimens were in a Carolina Micro Aquarium (#131016).
Subjects were easily acquired by “fishing” in my 23 year old 50 gallon Leiden-style aquarium. It is truly a “micro-menagerie”.
The “reverse UWOL” clip was shot with a Canon SD 1400 IS in a WP-DC37 underwater housing on a ProAm DVC200 crane (which was recommended by Per Johan Naesje- Thanks Per Johan!). I was not in the river but the tripod was. (Good thing because wading is illegal!) The sky was completely blown out and was rescued by the non replacement technique discussed at the end of Andrew Kramer’s Video Copilot Tutorial #42- Advanced Sky Replacement. I’m pleased how well the footage from the little camera cut against the other footage. I believe this is the only clip at normal speed.
Slow motion was done by:
1) Smooth Slow Record (120 fps) on a Sony HDR-HC9 conformed to 24P.
2) 60P conformed to 24P.
3) 30i conformed to 24P.
4) any of the above further slowed with Kronos.
Combination of 1 and 4 was least effective due to the reduced resolution. The fast motion was simply done in Premier.
Audio was a major concern for me. No Foley this time. What kind of sound does a paramecium make? I hope the choice of music worked.
Would any of these creatures really exist in a dewdrop? Obviously some are too large and the title is somewhat “creative license”, however, protists dry up and encyste and are quick to inhabit the most ephemeral of bodies of water. Even dewdrops…
As usual, all questions, comments and critique is welcomed. Thanks.